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cotH [2019-06-27 12:52:16]
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cotH [2019-06-27 12:52:16]

protein kinase, required for the assembly of several outer coat proteins
locus
BSU36060
pI
5.95
mw
42.65 kDa
protein length
362 aa Sequence Blast
gene length
1089 bp Sequence Blast
function
protection of [protein|EB690FA075EAC3E298C47AED082B5FC21E8F8F46|CotU] and [protein|9C59B51FC19FC83BD14272008BD441833DD1738E|CotC] in the mother cell
product
protein kinase
essential
no
synonyms
ywrH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.8|Not yet assigned]

    • Gene

    Coordinates
    3,716,009 → 3,717,097

    Phenotypes of a mutant

  • overexpression of ''cotH'' suppresses the phenotypes of a [gene|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE] mutant [Pubmed|24086406]
  • reduced [SW|germination] efficiency [Pubmed|27185916]

    • The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation with ATP and subsequent phosphorylation of [protein|2A0B2CA7C982C03C1AFBDB3540A918EE937B06CB|CotB] and [protein|49BAFE3E69F1F91AEF4154F55B44639DD3CE4A71|CotG] on serine residues [Pubmed|27185916]

    • Protein family

  • cotH family (according to Swiss-Prot)

    • [SW|Cofactors]

  • Mn2+ [Pubmed|27185916]

    • Structure

  • [PDB|5JD9] [Pubmed|27185916]

    • [SW|Localization]

  • inner spore coat

    • Expression and Regulation

    Operons

    genes
    [gene|D53067098F693669F61E5CCF6C06664580C40C79|cotH]-[gene|2A0B2CA7C982C03C1AFBDB3540A918EE937B06CB|cotB]-[gene|61004D2C1EDA3CD84514FD6D468BF594575616F4|ywrJ]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,8755863,1691789,12480901], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15383836], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

    • regulatory mechanism

  • [protein|0D7F5504590512E1598B94DC1734BF8FF67ED994|GerR]: activation, [Pubmed|20435725], in [regulon|0D7F5504590512E1598B94DC1734BF8FF67ED994|GerR regulon]
  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: repression, [Pubmed|15470121], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]
  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: activation, [Pubmed|26577401], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]

    • regulation

  • expressed late during sporulation in the mother cell ([SW|SigE], [protein|search|SigK], [protein|search|GerE], [SW|SpoIIID]) [Pubmed|26577401,15699190,8755863,15470121]
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    Biological materials

    Mutant

  • BKE36060 (Δ[gene|D53067098F693669F61E5CCF6C06664580C40C79|cotH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCATAATCCTCCTTACA, downstream forward: _UP4_TGAATGCGTGAAAATGGGTA
  • BKK36060 (Δ[gene|D53067098F693669F61E5CCF6C06664580C40C79|cotH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCATAATCCTCCTTACA, downstream forward: _UP4_TGAATGCGTGAAAATGGGTA

    • References

    Reviews

  • 27227299

    • Original Publications

  • 10198031,15699190,8755863,15470121,18065538,24086406,21984783,25259857,25115591,26484546,26953338,27185916,14762006,26577401,28870294